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SYBR® Green Extract-N-AmpTM組織PCR試劑盒
SYBR® Green Extract-N-AmpTM Tissue PCR Kit
| 產(chǎn)品編號(hào)： | 3246202 |
| 產(chǎn)品類別： | 試劑盒 |
| 純度規(guī)格： | sufficient for 1000 extractions, sufficient for 1000 amplifications |
| 包裝規(guī)格： | 1 KIT |
| 市場(chǎng)價(jià)： | 41290元 |
| 優(yōu)惠價(jià)： | 立即咨詢 |
SYBR® Green Extract-N-AmpTM組織PCR試劑盒
SYBR® Green Extract-N-AmpTM Tissue PCR Kit
基本信息
| NACRES | NA.55 |
| General description【一般描述】 | The SYBR® Green Extract-N-Amp™ Tissue PCR kit is a direct PCR (polymerase chain reaction) kit that contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva. |
| Application【應(yīng)用】 | SYBR® Green Extract-N-Amp™ tissue PCR kit has been used for DNA extraction and genotyping. |
| Features and Benefits【特點(diǎn)和優(yōu)勢(shì)】 |
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| Components【組分】 | 試劑盒含有Extraction Solution、Neutralization Solution、Tissue Preparation Solution和Extract-N-Amp SYBR Green PCR ReadyMix™。Extract-N-Amp SYBR Green PCR ReadyMix為2X反應(yīng)混合物，其中含有 SYBR Green、緩沖液、鹽、dNTP、Taq聚合酶和 JumpStart™ Taq 抗體。此試劑盒專為結(jié)合提取試劑使用進(jìn)行了優(yōu)化，其中含有適用于熱啟動(dòng)PCR、可增強(qiáng)特異性的 JumpStart Taq抗體，以及可用作實(shí)時(shí)定量PCR的非特異性報(bào)告標(biāo)志物的SYBR Green I。 |
| Principle【原理】 | DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances and the extract is ready for real-time PCR in any plate-based real-time thermal cycling system. An aliquot of the neutralized extract is then combined with the Extract-N-Amp™ SYBR® Green PCR ReadyMix™ and user-provided PCR primers. |
| Other Notes【其他說明】 | 更多信息，請(qǐng)見www.sigma-aldrich.com/extract-n-amp。 |
| Legal Information【法律信息】 | 本產(chǎn)品的使用受到如下一項(xiàng)或多項(xiàng)美國專利及其對(duì)應(yīng)的境外專利聲明保護(hù)：5,994,056和6,171,785。購買本產(chǎn)品，即相當(dāng)于依照境外的專利聲明獲得了一份受限制、不可轉(zhuǎn)讓的，僅此等數(shù)量的產(chǎn)品用于購買者內(nèi)部研究用途的的免責(zé)許可。其他專利聲明下的任何權(quán)利(如美國專利號(hào)6,814,934號(hào)下的設(shè)備和系統(tǒng)專利聲明)、進(jìn)行任何專利方法申請(qǐng)的權(quán)利、進(jìn)行任何形式的商業(yè)服務(wù)的權(quán)利，包括但不限于出于收費(fèi)或其他商業(yè)考慮而報(bào)告購買者的研究活動(dòng)結(jié)果的權(quán)利。本產(chǎn)品僅適合于研究用途。如需用于Roche專利許可的診斷用途，需另外征得Roche許可。有關(guān)購買許可的更多信息，可聯(lián)系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA獲取。 依照美國專利號(hào)5,338,671和5,587,287和其他地區(qū)對(duì)應(yīng)的專利，許可抗體用于體外研究用途。 Extract-N-Amp is a trademark of sigma-aldrich Co. LLC HOTSTART is a registered trademark of Molecular BioProducts, Inc. JumpStart is a trademark of sigma-aldrich Co. LLC ReadyMix is a trademark of sigma-aldrich Co. LLC SYBR is a registered trademark of Life Technologies |
產(chǎn)品性質(zhì)
| Quality Level【質(zhì)量水平】 | 200 |
| usage【用途】 | sufficient for 1000 amplifications sufficient for 1000 extractions sufficient for 1000 reactions |
| feature【特點(diǎn)】 | dNTPs included hotstart |
| technique(s) | PCR: suitable |
| web metadata keyword.default | XNATG |
| storage temp.【儲(chǔ)存溫度】 | −20℃ |
安全信息
| Pictograms【象形圖】 | GHS07,GHS08,GHS09 |
| Signal word【警示用語：】 | Danger |
| Hazard Statements | H315 - H317 - H319 - H334 - H335 - H411 |
| Precautionary Statements | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Classifications【危險(xiǎn)分類】 | Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3 |
| Target Organs【靶器官】 | Respiratory system |
| Storage Class Code【儲(chǔ)存分類代碼】 | 10 - Combustible liquids |
| WGK | WGK 3 |
